Plasmid_Backbone

Part:BBa_K209432:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)

pCEP4 with AarI A-D sites in MCS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6034
    Illegal EcoRI site found at 8330
    Illegal EcoRI site found at 8726
    Illegal XbaI site found at 609
    Illegal XbaI site found at 1146
    Illegal SpeI site found at 18
    Illegal SpeI site found at 2057
    Illegal PstI site found at 6786
    Illegal PstI site found at 8423
    Illegal PstI site found at 8816
    Illegal PstI site found at 10083
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6034
    Illegal EcoRI site found at 8330
    Illegal EcoRI site found at 8726
    Illegal NheI site found at 630
    Illegal NheI site found at 642
    Illegal SpeI site found at 18
    Illegal SpeI site found at 2057
    Illegal PstI site found at 6786
    Illegal PstI site found at 8423
    Illegal PstI site found at 8816
    Illegal PstI site found at 10083
    Illegal NotI site found at 646
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6034
    Illegal EcoRI site found at 8330
    Illegal EcoRI site found at 8726
    Illegal BglII site found at 604
    Illegal BglII site found at 10111
    Illegal BamHI site found at 745
    Illegal XhoI site found at 727
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6034
    Illegal EcoRI site found at 8330
    Illegal EcoRI site found at 8726
    Illegal XbaI site found at 609
    Illegal XbaI site found at 1146
    Illegal SpeI site found at 18
    Illegal SpeI site found at 2057
    Illegal PstI site found at 6786
    Illegal PstI site found at 8423
    Illegal PstI site found at 8816
    Illegal PstI site found at 10083
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6034
    Illegal EcoRI site found at 8330
    Illegal EcoRI site found at 8726
    Illegal XbaI site found at 609
    Illegal XbaI site found at 1146
    Illegal SpeI site found at 18
    Illegal SpeI site found at 2057
    Illegal PstI site found at 6786
    Illegal PstI site found at 8423
    Illegal PstI site found at 8816
    Illegal PstI site found at 10083
    Illegal NgoMIV site found at 733
    Illegal AgeI site found at 681
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 5402
    Illegal BsaI site found at 6960
    Illegal BsaI.rc site found at 4440
    Illegal BsaI.rc site found at 9660


Design Notes

Source

Parent vector is from Invitrogen.

References